Bacteriostatic Water

Peptides: independent lab report for each listed batch · Water: documentation varies by vial (see product page)

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Three formats for reconstitution — choose the one that fits your work. Specifications, comparison and FAQs below.

    Bacteriostatic Water USP 30mL sterile vial

    Bacteriostatic Water (Hospira)

    30 mL multi-dose vial

    Bacteriostatic Water (Hospira) is a sterile 30mL multi-dose vial preserved with 0.9% benzyl alcohol. It is the standard…

    Bacteriostatic Water 3mL research vial

    Bacteriostatic Water 3mL

    3 mL multi-use vial

    Multi-use vial. This 3 mL vial can be drawn from more than once. The 0.9% benzyl alcohol preservative inhibits…

    Batch BAC003-001
    Genetek Bacteriostatic Water, 10 mL single-use ampoule with 0.9% benzyl alcohol preservative

    Bacteriostatic Water (BAC Water) – 10 mL Ampoule

    10 mL single-use ampoule

    Single-use only — not a multi-use container. Each 10 mL plastic ampoule is opened by breaking off its top…

Bacteriostatic water is sterile, USP-grade water preserved with 0.9% benzyl alcohol — a bacteriostatic agent that inhibits microbial growth, so a sealed vial can be accessed repeatedly across a protocol rather than discarded after a single draw. It is the standard diluent for reconstituting lyophilized research peptides. Reserve Research stocks it in three sizes; each product page states what documentation is available for that vial. Supplied for laboratory research use only.

Choosing a vial size

— Hospira bacteriostatic water, 30 mL — the reference multi-use presentation, and the format most protocols are written around. Suited to reconstituting several compounds, or to longer runs drawn from one sealed vial.
— Bacteriostatic water, 3 mL — a single-compound format for bringing one lyophilized vial into solution without opening a larger vial.
— Bacteriostatic water, 10 mL ampoule — a single-use format: the plastic ampoule is opened by breaking off its top and cannot be resealed, so draw what you need in one session and discard the rest.

Specifications

— Composition: sterile, USP-grade water with 0.9% (9 mg/mL) benzyl alcohol as a bacteriostatic preservative
— Appearance: clear, colorless solution
— Presentation: sealed multi-use vial (30 mL, 3 mL) or single-use ampoule (10 mL)
— Storage: controlled room temperature, 20–25 °C (68–77 °F). Do not freeze. Keep the stopper sealed and protected from light.
— After first access: the benzyl alcohol content is what permits repeated draws from a sealed vial. Standard USP labeling specifies discarding the vial 28 days after it is first accessed.

Bacteriostatic water vs. preservative-free sterile water

The two are not interchangeable. Preservative-free sterile water contains no bacteriostatic agent — once the seal is broken, the contents are intended for a single use and any remainder is discarded. The 0.9% benzyl alcohol in bacteriostatic water suppresses microbial growth between draws, which is why it is the usual choice for reconstituting a peptide that will be sampled across a series of experiments rather than used in one preparation. Where a protocol specifies a preservative-free diluent, bacteriostatic water is not a substitute.

Reconstitution

Lyophilized peptides are brought into solution by introducing diluent slowly down the inner wall of the vial rather than directly onto the powder, then allowing the material to dissolve without agitation. Swirling is preferred to shaking, which can shear peptide chains. Reconstitution volume determines working concentration and is set by the protocol, not by the vial size — a larger diluent vial simply means fewer vials opened across a run.

Certificates of analysis

Documentation differs by vial. The 3 mL vial’s current batch (BAC003-001) has an independent KMD Analytical report covering appearance, HPLC purity and benzyl alcohol content (9.13 mg/mL, 101% of the 9 mg/mL label claim); that report does not assess sterility or endotoxin. The Hospira 30 mL vial and the Genetek 10 mL ampoule are manufacturer-sealed and have no separate Reserve Research lab report. Batch numbers with a posted report can be looked up on our COA verification page.

Common questions

Is bacteriostatic water the same as bacteriostatic sodium chloride?

No. Bacteriostatic sodium chloride is a preserved saline solution; bacteriostatic water contains no sodium chloride. Protocols specify one or the other and they are not interchangeable.

Why a 30 mL vial when a protocol needs only a few millilitres?

The multi-use format exists precisely so one sealed vial can serve many reconstitutions. The preservative is what makes that possible.

Does it need refrigeration?

No. Bacteriostatic water is stored at controlled room temperature. Reconstituted peptide solutions have their own storage requirements, which are compound-specific.

What does “0.9% benzyl alcohol” mean?

9 mg of benzyl alcohol per millilitre of water — the bacteriostatic agent, at the concentration specified by USP for this preparation.

Research use only

All products supplied by Reserve Research, bacteriostatic water included, are sold strictly for in-vitro laboratory research. They are not drugs, are not for human or veterinary use, and are not intended to diagnose, treat, cure or prevent any disease. See our Compliance & RUO Policy.

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